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Dr. Thomas Pesacreta
Director, Microscopy Center
Associate Professor of BIOLOGY
PO Box 42451
Lafayette, LA 70504
Phone: (337) 482-5233
Fax: (337) 482-5834
|Research Interests :|
|Atomic force microscopy of xylem pit membranes - Dicotyledons depend upon small, relatively porous structures in the vessel element wall to transfer water from vessel to vessel as it moves up the stem. These porous pit membranes are composed of carbohydrates. Until recently, it was believed that the pit membrane was a closely knit mass of fibers. Recently, I showed, using atomic force microscopy in water, that the fibers were arranged in a much more open pattern, one that would be consistent with the passage of water. The enigma that remains is that the surface of the pit membrane is apparently covered by some sort of non-fibrillar layer, and it is not yet clear how water could pass through it.|
|Location and physiological role of the plant cuticle and other possible hydrophobic wall layers within the leaf - Our recent work has shown the existence of a relatively thin plant cuticle on the inner tangential wall of epidermal cells where they are exposed to the substomatal cavity. Preliminary evidence suggests that this cuticle is a physiologically significant barrier to water, thus encapsulating the epidermis in a hydrophobic wall except where it is physically connected to the mesophyll. Current research involves fluorescence microscopy of microinjected or applied chemicals that bind to specific biochemical entities to confirm whether the epidermis, or any other cell types in the leaf are isolated from these dissolved chemicals.|
|Plant myosin isolation, characterization, and localization - We have sequenced several myosin clones from corn (Zea mays). Using antibodies generated against specific gene fragments, we have co-localized myosin to several organelles, and to a chaperonin protein. The interaction with the chaperonin is of interest, as well as the linkage between myosin and organelles. Frustrating these investigations is the high amount of protease activity that seems to co-purify with the myosin. It seems possible that the protease activity and the involvement with chaperonin are related so that myosin can be closely regulated.|
|Dr. Thomas Pesacreta||Microscopy Center|
|Director, USL Microscopy Center||University of Louisiana at Lafayette|
|Associate Professor||P.O. Box 42451|
|Graduate Faculty||Lafayette, LA 70504-2451|
|Office (337) 482-5233|
|Laboratory (337) 482-5769|
|Fax (337) 482-5834|
|Home (337) 235-4019|
|B.S., Agronomy, Virginia Polytechnic Institute and State University,
|Ph.D., Botany, Cornell University, Ithaca, 1982|
|Honors and Awards|
|Maria Moors Cabot Foundation Fellowship, Harvard University, 1985-1987|
|Dr. M.V. Parthasarathy, Cornell University, Section of Plant Biology, Ithaca, New York, 1977-1982|
|University of California, Davis, 1982-85, with Dr. W.J. Lucas,
Dept. of Botany
|Harvard University, Cambridge, Massachusetts, 1985-88, with Drs. D. Branton and D. Kiehart, Dept. of Cellular and Developmental Biology|
|Graduate Students at ULL (for which I am, or have been the committee head)|
|Durand, John M. - M.S., Biology 1993. Thesis title - Analysis
of structural and biochemical characteristics of stamens of Asteraceae.
|Guo, Jie - M.S. Biology 1996. Thesis title - Isolation of phosphatases
from the inner epidermis of onion bulb scales
|Liyun, Liu - Ph.D. candidate, Biology. Research topic - Molecular
biology of onion cytoskeletal proteins.
|Niandong, Liu - Ph.D program, Biology. Research topic - Analysis
of acid phosphatases in onion.
|Zhou, Juhua - Ph.D. program, Biology. Research topic - Myosin
|Zhengyang, Wan - M.S. program, Biology. Research topic - Myosin in corn.|
|Louisiana Educational Quality Support Fund, Characterization
and imunolocalization of plant myosin, 1989-1992, $81,000.00, PD.
|National Science Foundation, Cuticle elasticity and viscoelastic
properties of thistle staminal filaments, 1992-1993, $115,000.00, co-PI
with 3 others.
|Louisiana Educational Quality Support Fund, Integrated Engineering
of Injection Molding Processees, 1993-1995, $167,752.00, co-PI with 3 others.
|Louisiana Educational Quality Support Fund (equipment enhancement),
An atomic force microscope for materials science, 1994, $58,048.00, PD
|United States Dept. of Agriculture, Modeling of wood fiber
based composites using individual fiber properties, 1096-98, $86,000, co-PI
with one other.
|United States Forestry Service, Atomic force microscopy of
wood fibers. 1996-97, $5000.00. PI.
|United States Dept. of Agriculture, Analysis of cotton fiber surface structure by atomic force microscopy. 1997-98, $19,950.00. PI.|
|Refereed Publications (in chronological order)|
|Byrne, J.M., T.C. Pesacreta and J.A. Fox. 1977. Development
and structure of the vascular connection between the primary and secondary
root of Glycine max (L.) Merr. Amer. J. of Botany 64:946-959.
|Byrne, J.M., T.C. Pesacreta and J.A. Fox. 1977 Vascular pattern
change caused by a nematode, Meloidogyne incognita in the lateral roots
of Glycine max (L.) Merr. Amer J. of Botany, 64:960-965.
|Parthasarathy, M.V. and T.C. Pesacreta. 1980. Microfilaments
in plant vascular cells. Canadian J. of Botany 58(7):807
|Pesacreta, T.C., W.W. Carley, W.W. Webb, and M.V. Parthasarathy.
1982. F-actin in conifer roots. Proc. Natl. Acad. Sci. U.S.A. 79:2898-2901.
|Pesacreta, T.C. and M.V. Parthasarathy. 1984. Microfilament
bundles in the roots of a conifer, Chamaecyparis obtusa Protoplasma 125:
|Pesacreta, T.C. and M.V. Parthasarathy. 1984. Improved staining
of microfilament bundles in plant cells for high-voltage microscopy. J.
Microsc. 133(1): 73-77.
|Pesacreta, T.C. and W.J. Lucas. 1985. Presence of a partially
coated reticulum in angiosperms. Protoplasma 125: 173-184.
|Pesacreta, T.C. and W.J. Lucas. 1986. Presence of a membrane
coat and a coated vesicle-associated reticulum of membranes: their structure
and possible interrelationships in Chara corallina. J. Cell Biol. 98: 1537-1545.
|Pesacreta, T.C., A.B. Bennett and W.J. Lucas. 1986. Spectrophotometric
and cytochemical analyses of phosphatase activity in Beta vulgaris L.J.
Histochem. Cytochem. 34: 327-338.
|Lucas, W.J., D.W. Kiefer, and T.C. Pesacreta. 1986. Influence
of culture medium pH on charasome development and chloride transport in
Chara corallina. Protoplasma 130: 5-11.
|Thomas C. Pesacreta, Timothy J. Byers, Ronald Dubreuil, Daniel
P. Kiehart, and Daniel Branton. 1989. Drosophila spectrin: The membrane
skeleton during embryogenesis. J. Cell Biol. 108:1697-1709.
|Kiehart, D.P., A. Ketchum, P. Young, D. Lutz, T.C. Pesacreta,
S. Inoue, and T.-L. Chen. 1990. Contractile proteins in Drosophila development.
N.Y. Acad. Sci. 582:233- 251.
|P. Young, T.C.Pesacreta, and D.P. Kiehart. 1991. Cytoplasmic
myosin in Drosophila. Development 111:1-10.
|Marquez-Sterling, N., I.M. Herman, T. Pesacreta, H. Arai, G.
Terres, M. Forgac. 1991. Immunolocalization of the vacuolar type H-APTase
from clathrin coated vesicles. Eur. J. of Cell Biol. 56:19-33.
|Pesacreta, T.C., V.I. Sullivan, K.H. Hasenstein, and J.M. Durand.
1991. Thigmonasticity of thistle staminal filaments. I. Involvement of a contractile
cuticle. Protoplasma 121: 54-64.
|Pesacreta, T.C., V.I. Sullivan, and K.H. Hasenstein. 1993.
The connective base of Cirsium horridulum (Asteraceae): Description and comparison
with the viscoelastic filament. Amer. J. Bot. 80:411-418.
|Hasenstein, K.H., T.C. Pesacreta, V.I. Sullivan. 1993. Thigmonasticity
of thistle staminal filaments II. Mechano-elastic properties. Planta 190:58-68.
|Barnett, J.P. and T. C. Pesacreta. 1993. Handling longleaf
pine seeds for optimal nursery performance. Southern Jour. of Applied Forestry
|Pesacreta, T.C., V.I. Sullivan and M. DeVore. 1994. The connective
base and filament of Acicarpha tribuloides (Calyceraceae). Amer. J. Bot. 81:753-759.
|Sullivan, V.I., T.C. Pesacreta, J.Durand, and K.H. Hasenstein.
1994. A survey of autofluorescent patterns observed in the connective base
epidermis in species of Asteraceae. Amer. J. Bot. 81:1119-1127.
|Pesacreta, T.C. and T. Stuessy. 1996. Taxon 45:473-485. Autofluorescent
walls of connective bases in anthers of Barnadesioideae (Asteraceae) and systematic
|Pesacreta, T.C., Carlson, L.C., and Triplett, B.A. 1997. Atomic
force microscopy of cotton fiber cell wall surfaces in air and water:quantitative
and qualitative aspects. Planta 202:435-442.
|Guo, J. and Pesacreta, T.C. 1997. Purification and characterization
of an acid phosphatase from the bulb of Allium cepa L. var. Sweet Spanish.
J. of Plant Physiology (in press).
|Pesacreta, T.C. and K. H. Hasenstein 1999. The internal cuticle
of Cirsium horridulum (Asteraceae) leaves. American Journal of Botany 86:923-928.
|Zhou, J., T. C. Pesacreta and R.C. Brown 1999 RNA isolation
from oligosaccharide-rich tissues. Plant Molecular Biology Reporter 17:4.
|Liu, L., Zhou J. and Pesacreta, T.C. 2001 Maize myosins: Diversity,
localization and function. Cell Motility and the Cytoskelton 48:130-148.
|Marga, F., Pesacreta, T.C. and Hasenstein, K.H. 2001 Biochemical
analysis of elastic and rigid cuticles of Cirsium horridulum Planta 213:841-848.
|Farshad, F., Pesacreta T.C., Garber, J.D. and Bikki, S.R. 2001
A comparison of surface roughness of pipes as measured by two profilometers
and atomic force microscopy. Scanning 23:241-248.
|Farshad, Fred F. and Thomas C. Pesacreta 2003 Coated pipe interior
surface roughness as measured by three scanning probe instruments. Anti-Corrosion
Methods and Materials 50:6-16.
|Wang, Z., and TC Pesacreta 2004 A subclass of myosin XI is
associated with mitochondria, plastids, and the molecular chaperone subunit
in maize. Cell Motility and the cytoskeleton 57:218-232.
|H. J. Kim, T. C. Pesacreta, and B. A. Triplett 2004 Cotton-fiber
germin-like protein. II: Immunolocalization, purification, and functional
analysis. Planta 218: 525–535.
|Pesacreta, T.C., LH Groom and TG Rials. 2005. Atomic force microscopy of the intervessel pit membrane in the stem of Sapium sebiferum (Euphorbiaceae). IAWA Journal 26:397-426.|
|Pesacreta, T.C. and W.J. Lucas. 1986. Phosphatase activities
in Beta vulgaris leaves. In Phloem transport. Eds Cronshaw, Lucas and Giaquinta.
pp 135-144. A.Liss, N.Y.
|2. Pesacreta, T.C. and M.V. Parthasarathy. 1987. Developmental ultrastructure in gymnosperms. In Cell and tissue culture in forestry. Eds Bonga, J.M and D.J. Durzan. pp. 353-351. M. Nijhoff Dordrecht.|
|Most microscopy and associated preparative techniques, image analysis, immunocytochemistry, autoradiography, phosphatase and zinc-iodide histochemistry, SDS-PAGE electrophoresis, isoelectric focusing, gel filtration, ion exchange chromatography, western blotting, northern blotting, screening cDNA libraries, PCR, DNA sequencing, electroelution of proteins from gels, ultracentrifugation, immunization and bleeding of rabbits and mice, monoclonal antibody production, spectrophotometric assay of proteins, spectrophotometric assay of phosphatase and ATPase reaction products, protein isolation from brain, liver, corn, and onions, protein concentration, immunoprecipitations, solid-phase immunobinding assays, affinity purification of antibodies, thin-layer chromatography of oligosaccharides|
Document last revised Thursday, November 10, 2005 3:31 PM
© Copyright 2003 by the University of Louisiana at Lafayette
Electron Microscopy Center, P.O. Box 42451, Lafayette LA 70504
Billeaud Hall, Room 137 · 337/482-5769 · email@example.com